Detailed view for Tb927.7.1780

Basic information

TDR Targets ID: 13807
Trypanosoma brucei, Adenine phosphoribosyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.9517 | Length (AA): 235 | MW (Da): 25973 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00156   Phosphoribosyl transferase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0003999   adenine phosphoribosyltransferase activity  
GO:0009116   nucleoside metabolic process  
GO:0006166   purine ribonucleoside salvage  

Metabolic Pathways

Purine metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 235 5vn4 (A) 1 235 99.99 0 1 2.2266 -1.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 5VN4:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127379)

Species Accession Gene Product
Arabidopsis thaliana AT5G11160   adenine phosphoribosyltransferase 5
Arabidopsis thaliana AT4G22570   adenine phosphoribosyl transferase 3
Arabidopsis thaliana AT1G80050   adenine phosphoribosyl transferase 2
Arabidopsis thaliana AT1G27450   adenine phosphoribosyl transferase 1
Arabidopsis thaliana AT4G12440   adenine phosphoribosyl transferase 4
Brugia malayi Bm1_49565   Adenine phosphoribosyltransferase
Candida albicans CaO19.9023   likely phosphoribosyltransferase similar to S. cerevisiae APT1 (YML022W) which is involved in salvage pathways of adenine, hypox
Candida albicans CaO19.1448   likely phosphoribosyltransferase similar to S. cerevisiae APT1 (YML022W) which is involved in salvage pathways of adenine, hypox
Caenorhabditis elegans CELE_T19B4.3   Protein T19B4.3
Dictyostelium discoideum DDB_G0270174   adenine phosphoribosyltransferase
Dictyostelium discoideum DDB_G0287985   hypothetical protein
Drosophila melanogaster Dmel_CG18315   Adenine phosphoribosyltransferase
Escherichia coli b0469   adenine phosphoribosyltransferase
Echinococcus granulosus EgrG_000069100   adenine phosphoribosyltransferase
Echinococcus multilocularis EmuJ_000069100   adenine phosphoribosyltransferase
Giardia lamblia GL50803_112885   Adenine phosphoribosyltransferase
Homo sapiens ENSG00000198931   adenine phosphoribosyltransferase
Leishmania braziliensis LbrM.26.0130   adenine phosphoribosyltransferase
Leishmania donovani LdBPK_260120.1   adenine phosphoribosyltransferase
Leishmania infantum LinJ.26.0120   adenine phosphoribosyltransferase
Leishmania major LmjF.26.0140   adenine phosphoribosyltransferase
Leishmania mexicana LmxM.26.0140   adenine phosphoribosyltransferase
Loa Loa (eye worm) LOAG_12522   hypothetical protein
Loa Loa (eye worm) LOAG_11857   adenine phosphoribosyltransferase
Mus musculus ENSMUSG00000006589   adenine phosphoribosyl transferase
Mycobacterium tuberculosis Rv2584c   Adenine phosphoribosyltransferase Apt (APRT) (AMP diphosphorylase) (AMP pyrophosphorylase) (transphosphoribosidase)
Mycobacterium ulcerans MUL_1728   adenine phosphoribosyltransferase
Oryza sativa 4336327   Os04g0504000
Oryza sativa 4329972   Os02g0613900
Oryza sativa 4352670   Os12g0589100
Oryza sativa 4343246   Os07g0484800
Onchocerca volvulus OVOC743   Adenine phosphoribosyltransferase homolog
Saccharomyces cerevisiae YML022W   adenine phosphoribosyltransferase APT1
Schistosoma japonicum Sjp_0031570   ko:K00759 adenine phosphoribosyltransferase [EC2.4.2.7], putative
Schistosoma japonicum Sjp_0031590   ko:K00759 adenine phosphoribosyltransferase [EC2.4.2.7], putative
Schistosoma mansoni Smp_054410   Adenine phosphoribosyltransferase
Schistosoma mansoni Smp_054360   Adenine phosphoribosyltransferase
Schmidtea mediterranea mk4.040520.00   Adenine phosphoribosyltransferase
Schmidtea mediterranea mk4.003142.03   Adenine phosphoribosyltransferase
Schmidtea mediterranea mk4.041842.00  
Schmidtea mediterranea mk4.001237.02   Adenine phosphoribosyltransferase
Trypanosoma brucei gambiense Tbg972.7.1780   Adenine phosphoribosyltransferase, putative
Trypanosoma brucei Tb927.7.1780   Adenine phosphoribosyltransferase, putative
Trypanosoma congolense TcIL3000_0_32550   Adenine phosphoribosyltransferase, putative
Trypanosoma cruzi TcCLB.507519.140   Adenine phosphoribosyltransferase, putative
Trypanosoma cruzi TcCLB.508207.74   Adenine phosphoribosyltransferase, putative
Treponema pallidum TP1039   adenine phosphoribosyltransferase

Essentiality

Tb927.7.1780 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.1780 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.1780 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.1780 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.1780 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0469 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.7.1780 (Trypanosoma brucei), Adenine phosphoribosyltransferase, putative
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